Journal: Journal of Allergy

Article Title: How Can Microarrays Unlock Asthma?

doi: 10.1155/2012/241314

Figure Lengend Snippet: List of a number companies currently providing miRNA microarray technology.

Article Snippet: Ambion , mir Vana miRNA bioarrays V2 , http://www.ambion.com/.

Techniques: Microarray

Journal: Journal of Allergy

Article Title: How Can Microarrays Unlock Asthma?

doi: 10.1155/2012/241314

Figure Lengend Snippet: Overview of mir Vana miRNA bioarray methodology. Total RNA is extracted from tissue or cells and miRNA purified. Poly(A) polymerase is then added in the presence of modified dATPs and normal dATP. A poly(A) tail containing the modified dATPs is then added to all RNAs present in the sample. Fluorescent dye is added which binds to the poly(A) tail and the sample is hybridized to the array.

Article Snippet: Ambion , mir Vana miRNA bioarrays V2 , http://www.ambion.com/.

Techniques: Purification, Modification

Journal: Journal of Allergy

Article Title: How Can Microarrays Unlock Asthma?

doi: 10.1155/2012/241314

Figure Lengend Snippet: The GEO accession number for microarray studies conducted on asthma.

Article Snippet: Ambion , mir Vana miRNA bioarrays V2 , http://www.ambion.com/.

Techniques: Microarray, Expressing, Clone Assay, Infection, Functional Assay, Sequencing, Synthesized

Journal: PLoS ONE

Article Title: Muscarinic receptor activation in colon cancer selectively augments pro-proliferative microRNA-21, microRNA-221 and microRNA-222 expression

doi: 10.1371/journal.pone.0269618

Figure Lengend Snippet: A) miRNA microarray profiles were performed on RNA extracted from HT-29 cells treated with vehicle or 100 μM ACh for 4 h. Data were normalized using the global Lowess regression algorithm and are expressed as log base 2 transformed ratios of the sample signal to the control reference pool signal. miRNAs with statistically significant changes (p<0.05) after ACh treatment are shown in the heat map; red represents increased expression compared to the pooled reference control, and green represents decreased expression. B) Changes in miR-21, miR-221, and miR-222 expression were confirmed by qPCR in HT-29 and H508 human colon cancer cells. Results are means ± SE, n = 4. *, indicates p<0.05 for the test sample compared to control. C) Expression of miR-21, miR-221, and miR-222 in sporadic colonic cancers and adjacent normal-appearing tissue were measured by microarray. Results are means ± SE, n = 4. *, indicates p<0.05 for expression in cancer compared to normal.

Article Snippet: Human miRNA profiles were analyzed using Exiqon miRNA array 7th Gen (Product #208500) for HT-29 cells and mirVana miRNA Bioarrays v. 2 (Thermo Fisher Scientific) for human colon samples according to the manufacturer’s instructions.

Techniques: Microarray, Transformation Assay, Control, Expressing

Journal: PLoS ONE

Article Title: Muscarinic receptor activation in colon cancer selectively augments pro-proliferative microRNA-21, microRNA-221 and microRNA-222 expression

doi: 10.1371/journal.pone.0269618

Figure Lengend Snippet: A) Pri-miR-21 and B) pri-miR-221/222 levels were measured by qPCR in HT-29 cells. Cells were pre-incubated for 45 min with 5 μM atropine to inhibit M 3 R activation, 5 μM Gӧ6976 to inhibit PKC activation, and 10 μM SB203580 (SB203) to inhibit p38 activation. Then, 100 μM ACh was added for an additional 60-min incubation. Bars represent means ± SEM. n = 4. *, indicates p< 0.05 compared to 100 μM ACh alone. C) HT-29 cells were pre-incubated for 45 min with 5 μM atropine before adding 100 μM ACh or transfecting cells with miR-222 mimics for an additional 24 h. Cell proliferation was measured using the WST-1 assay. Bars represent means ± SEM. n = 4 *, P < 0.05 compared to cells without atropine. D) Working model for post-M 3 R signal transduction mediating muscarinic receptor agonist-induced increases in selected miRNAs in colon cancer. The agonists, inhibitors, and miRNA mimics used to test each step in the proposed signaling pathway are shown.

Article Snippet: Human miRNA profiles were analyzed using Exiqon miRNA array 7th Gen (Product #208500) for HT-29 cells and mirVana miRNA Bioarrays v. 2 (Thermo Fisher Scientific) for human colon samples according to the manufacturer’s instructions.

Techniques: Incubation, Activation Assay, WST-1 Assay, Transduction